Nested PCR-RFLP is a high-speed method to detect fungicide-resistant Botrytis cinerea at an early growth stage of grapes.
Identifieur interne : 000752 ( Main/Exploration ); précédent : 000751; suivant : 000753Nested PCR-RFLP is a high-speed method to detect fungicide-resistant Botrytis cinerea at an early growth stage of grapes.
Auteurs : Seiya Saito [Japon] ; Shunji Suzuki ; Tsutomu TakayanagiSource :
- Pest management science [ 1526-498X ] ; 2009.
Descripteurs français
- KwdFr :
- Benzimidazoles (pharmacologie), Botrytis (effets des médicaments et des substances chimiques), Botrytis (génétique), Botrytis (isolement et purification), Fongicides industriels (pharmacologie), Maladies des plantes (microbiologie), Phényl-carbamates (pharmacologie), Polymorphisme de restriction (MeSH), Réaction de polymérisation en chaîne (méthodes), Résistance des champignons aux médicaments (MeSH), Vitis (croissance et développement), Vitis (microbiologie).
- MESH :
- croissance et développement : Vitis.
- effets des médicaments et des substances chimiques : Botrytis.
- génétique : Botrytis.
- isolement et purification : Botrytis.
- microbiologie : Maladies des plantes, Vitis.
- méthodes : Réaction de polymérisation en chaîne.
- pharmacologie : Benzimidazoles, Fongicides industriels, Phényl-carbamates.
- Polymorphisme de restriction, Résistance des champignons aux médicaments.
English descriptors
- KwdEn :
- Benzimidazoles (pharmacology), Botrytis (drug effects), Botrytis (genetics), Botrytis (isolation & purification), Drug Resistance, Fungal (MeSH), Fungicides, Industrial (pharmacology), Phenylcarbamates (pharmacology), Plant Diseases (microbiology), Polymerase Chain Reaction (methods), Polymorphism, Restriction Fragment Length (MeSH), Vitis (growth & development), Vitis (microbiology).
- MESH :
- chemical , pharmacology : Benzimidazoles, Fungicides, Industrial, Phenylcarbamates.
- drug effects : Botrytis.
- genetics : Botrytis.
- growth & development : Vitis.
- isolation & purification : Botrytis.
- methods : Polymerase Chain Reaction.
- microbiology : Plant Diseases, Vitis.
- Drug Resistance, Fungal, Polymorphism, Restriction Fragment Length.
Abstract
BACKGROUND
Grey mould caused by the fungus Botrytis cinerea Pers. ex Fr. is one of the major diseases in grapes. The use of fungicides is a simple strategy to protect grapes against B. cinerea disease. However, phenotypes exhibiting resistance to fungicides have been detected in B. cinerea populations. The variation of fungicide-resistant B. cinerea isolates renders B. cinerea disease control difficult in grapevine fields.
RESULTS
The authors have developed a nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect fungicide-resistant B. cinerea isolates at an early growth stage of grapes in grapevine fields. The nested PCR-RFLP method was carried out to detect benzimidazole-, phenylcarbamate- and/or dicarboximide-resistant B. cinerea isolates from grape berries and leaves at Eichorn-Lorenz growth stage 25 to 29. This method successfully detected fungicide-resistant B. cinerea isolates at an early growth stage of grapes. In addition, only 8 h was required from tissue sampling to phenotyping of fungicide resistance of the isolates.
CONCLUSION
It is proposed that the early diagnosis of fungicide-resistant B. cinerea isolates would contribute to further improvement of integrated pest management against B. cinerea in grapevine fields, and that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for this purpose.
DOI: 10.1002/ps.1668
PubMed: 19051204
Affiliations:
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Le document en format XML
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wicri:level="1"><nlm:affiliation>Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005</wicri:regionArea><wicri:noRegion>Yamanashi 400-0005</wicri:noRegion></affiliation></author><author><name sortKey="Suzuki, Shunji" sort="Suzuki, Shunji" uniqKey="Suzuki S" first="Shunji" last="Suzuki">Shunji Suzuki</name></author><author><name sortKey="Takayanagi, Tsutomu" sort="Takayanagi, Tsutomu" uniqKey="Takayanagi T" first="Tsutomu" last="Takayanagi">Tsutomu Takayanagi</name></author></analytic><series><title level="j">Pest management science</title><idno type="ISSN">1526-498X</idno><imprint><date when="2009" 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(microbiologie)</term><term>Phényl-carbamates (pharmacologie)</term><term>Polymorphisme de restriction (MeSH)</term><term>Réaction de polymérisation en chaîne (méthodes)</term><term>Résistance des champignons aux médicaments (MeSH)</term><term>Vitis (croissance et développement)</term><term>Vitis (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Benzimidazoles</term><term>Fungicides, Industrial</term><term>Phenylcarbamates</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Botrytis</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Botrytis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Botrytis</term></keywords><keywords scheme="MESH" qualifier="growth & development" 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The use of fungicides is a simple strategy to protect grapes against B. cinerea disease. However, phenotypes exhibiting resistance to fungicides have been detected in B. cinerea populations. The variation of fungicide-resistant B. cinerea isolates renders B. cinerea disease control difficult in grapevine fields.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The authors have developed a nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect fungicide-resistant B. cinerea isolates at an early growth stage of grapes in grapevine fields. The nested PCR-RFLP method was carried out to detect benzimidazole-, phenylcarbamate- and/or dicarboximide-resistant B. cinerea isolates from grape berries and leaves at Eichorn-Lorenz growth stage 25 to 29. This method successfully detected fungicide-resistant B. cinerea isolates at an early growth stage of grapes. In addition, only 8 h was required from tissue sampling to phenotyping of fungicide resistance of the isolates.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>It is proposed that the early diagnosis of fungicide-resistant B. cinerea isolates would contribute to further improvement of integrated pest management against B. cinerea in grapevine fields, and that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for this purpose.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19051204</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1526-498X</ISSN><JournalIssue CitedMedium="Print"><Volume>65</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Pest management science</Title><ISOAbbreviation>Pest Manag Sci</ISOAbbreviation></Journal><ArticleTitle>Nested PCR-RFLP is a high-speed method to detect fungicide-resistant Botrytis cinerea at an early growth stage of grapes.</ArticleTitle><Pagination><MedlinePgn>197-204</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/ps.1668</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Grey mould caused by the fungus Botrytis cinerea Pers. ex Fr. is one of the major diseases in grapes. The use of fungicides is a simple strategy to protect grapes against B. cinerea disease. However, phenotypes exhibiting resistance to fungicides have been detected in B. cinerea populations. The variation of fungicide-resistant B. cinerea isolates renders B. cinerea disease control difficult in grapevine fields.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The authors have developed a nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect fungicide-resistant B. cinerea isolates at an early growth stage of grapes in grapevine fields. The nested PCR-RFLP method was carried out to detect benzimidazole-, phenylcarbamate- and/or dicarboximide-resistant B. cinerea isolates from grape berries and leaves at Eichorn-Lorenz growth stage 25 to 29. This method successfully detected fungicide-resistant B. cinerea isolates at an early growth stage of grapes. In addition, only 8 h was required from tissue sampling to phenotyping of fungicide resistance of the isolates.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">It is proposed that the early diagnosis of fungicide-resistant B. cinerea isolates would contribute to further improvement of integrated pest management against B. cinerea in grapevine fields, and that the nested PCR-RFLP method is a high-speed, sensitive and reliable tool for this purpose.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Seiya</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, Kofu, Yamanashi 400-0005, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Shunji</ForeName><Initials>S</Initials></Author><Author 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MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>12</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>12</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19051204</ArticleId><ArticleId IdType="doi">10.1002/ps.1668</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Suzuki, Shunji" sort="Suzuki, Shunji" uniqKey="Suzuki S" first="Shunji" last="Suzuki">Shunji Suzuki</name><name sortKey="Takayanagi, Tsutomu" sort="Takayanagi, Tsutomu" uniqKey="Takayanagi T" first="Tsutomu" last="Takayanagi">Tsutomu Takayanagi</name></noCountry><country name="Japon"><noRegion><name sortKey="Saito, Seiya" sort="Saito, Seiya" uniqKey="Saito S" first="Seiya" last="Saito">Seiya Saito</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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